The Workshop will focus on the application of paramagnetic NMR, 13C direct detection methods and on solution structures of metalloproteins.
Participants will be trained at the spectrometers, working with challenging systems such as proteins involved into the FeS cluster assembly machinery. They will acquire experience in the most recent NMR techniques specifically developed for paramagnetic metalloproteins and for 13C direct detection. Participants can bring their own samples.
The school is open to PhD students, postdocs and Early Career Investigators. A maximum number of 20 participants will be considered.
The registration fee is 80 euros. It includes the participation in all the sessions, coffee breaks, lunches, shuttle bus transfer from Hotel, participation to social activities between the 25th and 26th of June. For participants attending also the Training Course “Fundamentals of magnetic resonance spectroscopies and metal trafficking” a comprehensive registration fee of 150 € is applied.
If you are a member of a laboratory affiliated with the TIMB3 Consortium you are eligible for support that will cover your registration and expenses. If you wish to apply for this support please submit a letter addressed to the course organizers indicating your current affiliation and outlining how your current research interests match the mission of the TIMB3 project, together with your application.
Morning lectures, Chair – Simone Ciofi Baffoni
9.00-10.30 - Mario Piccioli (Univ. Florence): Paramagnetic NMR, the toolkit
10.30-11.0 - Coffee Break
11.00-12.30 - Roberta Pierattelli (Univ. Florence): 13C direct detection for biomolecular NMR
14.00-17.45 - Parellel session
Mario Piccioli (Univ. Florence): Fast acquisition and 1D NOEs
15.45-16.00 Coffee Break
13C direct detection
Marco Schiavina and Isabella C. Felli (Univ. Florence): Multi dimensional 13C observed experiments: recovering the lost information
18.00 Transfer to Florence, Aperitif-dinner in Oltrarno
21.30 Sunset at Piazzale Michelangelo
Morning lectures, Chair – Mario Piccioli
9.00-10.00 - Francesca Cantini (Univ. Florence): NMR structures of paramagnetic molecules
10.00-11.00 - Michele Invernici (Univ. Florence): From relaxation rates to upper distance limits
11.00-11.30 Coffee Break
11.30-16.00 - Francesca Cantini (Univ. Florence): NMR assignment, structure calculations and overview
Mario Piccioli (Univ. Florence): IR-HSQC-AP, R2-weighted-HSQC-AP and other (hopefully) fancy experiments
16.00-16.15 Coffee Break
16.15-to the end - Let’s try with your samples
Anytime - Closure