Training School – Fundamentals of magnetic resonance spectroscopies and metal trafficking

The school will cover few methodological and applied aspects of NMR, EPR and bioinformatics, with a specific focus on metalloproteins.

Practical sessions will be performed using the NMR and EPR instrumentations and the other facilities available at CERM/CIRMMP infrastructure.
The school is open to PhD students, postdocs and Early Career Investigators. A maximum number of 20 participants will be considered.

Registration

Applicants are invited to submit the application by submitting their CV and list of publications, before February 29th, at nmrtraining2020@cerm.unifi.it

The registration fee is 100 euros. It includes the participation in all the sessions, coffee breaks, lunches, shuttle bus transfer from Hotel, participation to social activities between the 22nd and 24th of June. For participants attending also the Hand-on Workshop “NMR of paramagnetic proteins and applications” a comprehensive registration fee of 150 € is applied.

If you are a member of a laboratory affiliated with the TIMB3 Consortium you are eligible for support that will cover your registration and expenses. If you wish to apply for this support please submit a letter addressed to the course organizers indicating your current affiliation and outlining how your current research interests match the mission of the TIMB3 project, together with your application.

Program

Monday 22nd

8.30-9.00 - Registration

9.00-9.15 - Introduction

Morning lectures, Chair – Mario Piccioli

9.15-11.00 - Lucia Banci (Univ. Florence): Interactomics via NMR, Systems biology

11.00-11.20 Coffee Break

11.20-13.00 - Enrico Luchinat (Univ. Florence): In cell NMR

13.00-14.30 Lunch

Afternoon Practicals*

14.30-16.15 - In cell NMR Spectroscopy
Wetlab experience and sample preparation
Tutors: Letizia Barbieri (CIRMMP, Florence), Enrico Luchinat (Univ. Florence), Matteo Cremonini (Univ. Florence)

14.30-16.15 - Interactomics by NMR
Chemical exchange and metal-protein interactions
Tutors: Francesca Cantini (Univ. Florence), Simone Ciofi-Baffoni (Univ. Florence)

16.15-16.45 Coffee Break

16.45-18.30 - In cell NMR Spectroscopy
Spectra acquisition, with and without metals
Tutors: Letizia Barbieri (CIRMMP, Florence), Enrico Luchinat (Univ. Florence), Matteo Cremonini (Univ. Florence)

16.45-18.30 - Interactomics by NMR
Protein-Protein Interactions
Tutors: Francesca Cantini (Univ. Florence), Simone Ciofi-Baffoni (Univ. Florence)

18.30 CERM BBQ Party

Tuesday 23rd

Morning lectures, Chair – Simone Ciofi Baffoni

9.15-11.00 - Moreno Lelli (Univ. Florence): Solid state NMR of paramagnetic metalloproteins

11.00-11.20 Coffee Break

11.20-13.00 - Marilena Di Valentin (Univ. Padua): Advanced Pulse EPR Techniques for the Characterization of Metalloproteins

13.00-14.30 Lunch

Afternoon Practicals*

14.30-16.00 - EPR
EPR of metalloproteins
Tutors: Alessio Bonucci (CNRS Marseille), Francesco Torricella (Univ. Florence), Marilena Di Valentin (Univ. Padua)

14.30-16.00 - Solid State NMR
How to prepare a rotor
Tutors: Linda Cerofolini (CIRMMP, Florence), Marco Fragai (Univ. Florence), Stefano Giuntini (Univ. Florence)

16.00-16.30 Coffee Break

16.30-18.00 - EPR
DEER Experiments
Tutors: Alessio Bonucci (CNRS Marseille), Fabio Calogiuri (CIRMMP, Florence), Francesco Torricella (Univ. Florence)

18.00 - Transfer to Florence, free time downtown

Wednesday 24th

Morning lectures, Chair – Mario Piccioli

9.15-11.00 - Antonio Rosato (Univ. Florence): Bioinformatics of MetalloProteins

11.00-11.20 Tea/Coffee Break

11.20-13.00 - Claudio Luchinat (Univ. Florence): Conformational dynamics- Maximum occurrence analysis

13.00-14.30 Lunch

Afternoon Practicals*

14.30-16.00 - Bioinformatics
Metal Predator searching for metalloproteins
Tutors:Claudia Andreini(Univ. Florence), Valeria Putignano (Univ. Florence)

14.30-16.00 - Conformational Dynamics
MaxOcc
Tutors: Enrico Ravera (Univ. Florence), Lucia Gigli (Univ. Florence), Denise Selegato (Univ. Florence)

16.00 - Transfer to Florence, The Calcio Storico Fiorentino Experience

22.00 - Fireworks

*For each practical sessions, students will choose one of the two practicals that will be run in parallel, up to a maximum number of 12 participants per practical.